Mosquitocidal activity of  Anacardium occidentale ,  Afromomum melegueta ,  Garcina kola  and  Citrus sinensis  against the developmental stages of mosquito,  Anopheles gambiae  Giles

K.D. Ileke<sup>1</sup>; O. J.  Afolabi<sup>2</sup>; O.C. Ogungbite<sup>2</sup>; J.O. Olayinka-Olagunju<sup>1</sup>; O.M. Akanbi <sup>1</sup>

Mosquitocidal activity of Anacardium occidentale, Afromomum melegueta, Garcina kola and Citrus sinensis against the developmental stages of mosquito, Anopheles gambiae Giles

K.D. Ileke¹

, O. J. Afolabi²

, O.C. Ogungbite²

, J.O. Olayinka-Olagunju¹

, O.M. Akanbi ¹

1 Department of Environmental Biology and Fisheries, Faculty of Science, Adekunle Ajasin University, P.M.B 001, Akungba Akoko, Ondo State, Nigeria
2 Department of Biology, School of Science, Federal University of Technology, P.M.B 704, Akure, Ondo State, Nigeria

Author

Correspondence author
Journal of Mosquito Research, 2014, Vol. 4, No. 3 doi: 10.5376/jmr.2014.04.0003
Received: 19 Feb., 2014 Accepted: 03 May, 2014 Published: 24 Jun., 2014

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Preferred citation for this article:

Ileke et al., 2014, Mosquitocidal activity of Anacardium occidentale, Afromomum melegueta, Garcina kola and Citrus sinensis against the developmental stages of mosquito, Anopheles gambiae Giles, Journal of Mosquito Research, Vol.4, No.3 21-26 (doi: 10.5376/jmr.2014.01.0003)

Abstract

The insecticidal potential of Anacardium occidentale, Afromomum melegueta, Garcina kola and Citrus sinensis plants were tested against malaria vector, mosquito, Anopheles gambiae in the laboratory at ambient temperature of (28±2)℃ and relative humidity of (75±5)%. The oils of the four plants were extracted with hexane and they were prepared at concentration of 0.1%, 0.2%, 0.3%, 0.4% and 0.5%. The larvae and pupae of An. gambiae Giles were exposed to these concentrations of the plant oils for 24 hours and mortality was recorded at this periods. At all levels of concentrations, mortality of both the larvae and pupae of this insect increased with increase in the concentrations regardless the type of plant extract used. However, A. occidentale oil extract showed more insecticidal effect on both the larvae and pupae of An. gambiae at all concentrations but its effect was not significantly (p>0.05) different from oil of A. melegueta and C. sinensis at 0.1% to 0.3% concentrations. The oil extract of G. kola showed the lowest mortality effect on both the larvae and pupae of the insect at all concentrations but the effect was significantly (p<0.05) different from the control. It was observed that the larvae of An. gambiae were more susceptible to the oil extracts of all the plants tested. All the plants extracts used in this work showed high potency to larval and pupal mortality and could consequently be used to reduce prevalence of malaria in the endemic areas.

Keywords

Mosquito; Malaria; Anacardium occidentale; Afromomum melegueta; Garcina kola; Citrus sinensis

Many developing countries of the world including Nigeria have been noted for high prevalence of malaria disease among their citizens. Nigeria being the most populous country in Africa, more than 50% of her citizens is suffering from at least one episode of malaria every year. Ogungbamigbe et al. (2005) reported that in Nigeria, malaria disease kills more than 25% of her young once and thereby imposing great burden on the country in terms of loss in outputs and cost of treatments. Malaria disease is caused by protozoan parasites of the genus Plasmodium transmitted by the female anopheles mosquitoes. Mosquitoes transmit disease to more than 700 million people annually in Africa, South America, Central America, and much of Asia, with Africa being the most affected continent (WHO, 2010; Akinkurolere et al., 2011). WHO (2010) reported that the primary public health intervention for reducing malaria transmission at the community level is through vector control. It is the only intervention that can reduce malaria transmission from very high levels to close to zero (Akinkurolere et al., 2011).

Over the years, the control of this infamous vector insect has overwhelmingly relied on the use of synthetic chemical insecticides such as organophosphates, organochlorines and carbamates. In spite of the success of many of these synthetic chemical insecticides, they have many cons that are associated with their use; these include pest resurgence and resistance, lethal effects on non-target organisms, adverse effect on both human and environmental health (Akinkurolere et al., 2011; Ileke and Olotuah, 2012; Ashamo et al., 2013). The public alertness of these adverse effects of synthetic chemical insecticides is impeding their widespread use in the control of insects. Therefore, to make an improvisation to the use of these chancy synthetic chemical insecticides, a lot of research activities have been concentrated on the plant kingdom as new thoroughfare of controlling insect pests. Prior to the discovery and commercial success of the synthetic chemical insecticides in the late 1930s and early 1940s, botanical base insecticides have remained important weapon in the farmers armory in managing insect pest of their farm produce (Forim et al., 2012).

Many botanicals have proved their efficacy as insecticides against a wide range of insects and they are believed to be ecofriendly because they are readily biodegradable and target specific. Tropical countries of the world including Nigeria are well endowed with plants having insecticidal properties (Akinkurolere et al.,2006). Until now, despite the effectiveness of many botanical powders and extracts, their insecticidal activity is yet to be comparable to many synthetic chemical insecticides and the once that are believed to be comparable with chemical insecticides have not commanded more than 1% of the global insecticide market (Isman, 2000; Begum et al., 2013) because of their loss of potency over time. Therefore, there is a need to search for other plants that could potentially contend with chemical insecticides in their action. In responding to Nigerian government’s quest to fight against malaria disease in the country, this study investigated the potential of A. occidentale, C. sinensis, A. melegueta and G. kola against the developmental stages of malaria vector, Anopheles mosquitoes.

Discussion

Plant materials have received more attention since government of some countries have started restricting the use of many synthetic chemical insecticides because of their adverse effect on both human and their environment (Isman, 2000). However, plant base insecticides have been noted to have different effects on insects based on the type of botanical used, part of the plant used, solvent used for the extraction and types of phytochemical compounds present in the plant (Jeyabalan et al., 2003). Jenson et al. (2006.) reported that crude plant extracts are highly efficient for the control of mosquitoes, rather than the purified compounds.

The result obtained in this work showed that plant extracts have significant effect on the mortality of the An. gambiae larvae and pupae compared to the controls. Oil extract of A. occidentale showed the highest mortality of the insect larvae and pupae throughout the period of exposure. The high mortality of the insect larvae and pupae may be attributed to the active compounds presents in this plant. A. occidentale have been reported to contain anacardic acid and cardinal, quercetin, kaempferol, glycosides, triacylglycerols, fatty acids, several unsaponifiable compounds, triterpene, alcohols, sterols and tocopherols (Oliver-Bever, 1986; Rehm and Espig, 1991; Ileke and Olotuah, 2012). All these compounds have high mortality effect against insect larvae (Philipson and Wright, 1991). The oil extract of C. sinensis and A. melegueta also had a high mortality against both larvae and pupae of An. gambiae and this could also be associated with the type of secondary metabolite present in these plants. Toxicity of Citrus sinensis can be attributed to strong choky odour disrupting respiratory activity of the insect (Ileke et al., 2013).

At all levels of concentrations and periods of exposure, the larvae of An. gambiae were more susceptible to all the plant extracts than their pupae. The high mortality effect by these oil extracts could be related to their ability to block the spiracles through which the larvae of this insect breathe (Kaufmann and Briegel, 2004). Therefore, since the larvae of this insect depend solely on their spiracles for breathing, blockage of the spiracle by these oil extracts could lead to asphyxiation and subsequent death of the larvae (Akinkurolere et al.,2006, Ileke and Oni, 2011; Ileke and Olotuah, 2012). The high mortality recorded for the larvae of this insect than its pupae could also be related to the active feeding of the larvae since pupae stages of the insect are not feeding. During feeding, the larvae must have ingested some active compounds in the oil extract of the plants, thereby leading to stomach poisoning. The findings of this work were in accordance with the result of Al-Dakhil and Morsy (1999), Amusan and Okorie (2002), El-Bokl (2003), Nathan et al. (2005) as well as (Akinkurolere et al. (2011) in which plant oils were found effective against mosquito larvae and pupae. From the results obtained in this research, the effectiveness of the plant extracts could be graded as A. occidentale>S. citirus>A. melegueta>G. kola.

Treatment of diseases is not as important as controlling the vector of the disease. In many developing countries like Nigeria where malaria disease is still responsible for the morbidity and mortality of about three million of both their old and young citizens, high level of mosquito breeding has be the major concern (Olasehinde et al., 2010). Also, high level of illiteracy and financial problem had also been a major obstacle in screening out mosquito because many Nigerians could not afford good accommodation (Akinkurolere et al.,2011).

Conclusion

In joining the Nigeria government in their combat against malaria disease and bearing in mind the importance of botanical base insecticides, high level of illiteracy and financial problem of many Nigerians, this research has been able to show the high efficacy of oil extracts of A. occidentale, S. citirus, A. melegueta and G. kola against An. gambiae larvae and pupae. The problem of finance may not be a major obstacle since these plants are readily available to both poor and the riches.

Results

Tables 1-5 present the mortality effect of different plant extracts on Anopheles gambiae at different concentrations. The mortality of An. gambiae larvae and pupae increased with increase in plants extracts concentration. At all concentrations, oil extract of A. occidentale recorded the highest larvae and pupae mortality and its effect was not significantly (p>0.05) different from other plant extracts. The effect of oil extract of A. occidentale was significantly (p<0.05) different from the extract of G. kola which recorded the lowest larvae and pupae mortality of An. gambiae at all the concentrations used. However, at 0.4% concentration effect of A. occidentale on An. gambiae larvae was significantly (p<0.05) different from all other plants extracts. None of the plants extracts was able to cause 100% larvae and pupae mortality except at 0.5% concentration at which the oil extract of A. melegueta, A. occidentale and S. citirus were able to cause complete mortality of An. gambiae larvae mortality.

Table 1 Percentage mortality of Anopheles gambiae at 24 hours post treatment with 0.1% of plant extracts

Table 2 Percentage mortality of Anopheles gambiae at 24 hours post treatment with 0.2% of plant extracts

Table 3 Percentage mortality of Anopheles gambiae at 24 hours post treatment with 0.3% of plant extracts

Table 4 Percentage mortality of Anopheles gambiae at 24 hours post treatment with 0.4% of plant extracts

Table 5 Percentage mortality of Anopheles gambiae at 24 hours post treatment with 0.5% of plant extracts

Materials and Methods

Collection of plant materials

The nuts of A. occidentale, seeds of A. melegueta, G. kola and peels of C. sinensis were bought at Oba market, Akure, Ondo State, Nigeria. The collected plant materials were taken to Research Laboratory, Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko, Ondo state for authentication. The plant materials were air dried in the laboratory and milled into fine powders and were kept inside different covered plant containers until further use.

Extraction of plant materials

The oil extracts of the plant materials were made by soaking 200g of each powder in 2litres of n-hexane and heated in water bath at 60^oC for 1h and decanted. The mixtures were stirred occasionally with a glass rod. The resulting mixture was filtered using a double layer of Whatman No. 1 filter paper and the solvent evaporated using a rotary evaporator at 30 to 40^oC with rotary speed of 3 to 6 rpm for 8 hours (Udo 2011). Each of the extracted oils was kept inside covered containers for subsequent use.

Mosquito baits, consisting of shallow containers with a large surface area were established under a partial shade in an open field. The container was filled with rain water to mimic mosquito natural breeding environment and to attract adult female for oviposition. Small quantity of industrial yeast was sprinkled on the surface of the water and allowed to decompose slowly to nourish the developing larvae. Wild mosquitoes were allowed to freely visit the baits and to lay eggs and the baits were later transferred to the laboratory where larvae and pupae were identified using stand methods and maintained at temperature of (28±2)℃, (75±5)% and 14:10 L:D relative humidity.

Effect of plant extracts on larvae and pupae of An. gambiae

Larvicidal and pupacidal activity of the plant extracts was carried out at different concentrations by preparing the required stock solutions following the standard procedure described by WHO (1996). The desired concentrations were achieved by adding 1.0 μg of crude extract of any of the four plant extracts to 100 ml of de-chlorinated water. From this, five concentrations of 0.1, 0.2, 0.3, 0.4 and 0.5% of each plant extracts were prepared. The extracts were mixed with water in a beaker at the desired concentration in the presence of small amount of yeast powder to serve as food source for the larvae. Then 10 larvae and pupae of An. gambiae were introduced into separate beaker. Beakers containing only water were set as control and were also infested with larvae and pupae of the insect. There were three replicates for each concentration and the control. Mortality was observed over 24 h, after which the larvae and pupae were introduced into distilled water to notice recovery. A recovery time of 5 minutes was allowed (WHO, 1996). The larval mortality in treatments was corrected for the controls (Abbott, 1925). Larvae and pupae were counted as dead when they were not coming to the surface for respiration and were insensitive to probe (Sivagnaname and Kalyanasundaram, 2004; Akinkurolere et al., 2011).

Data analysis

Data were subjected to analysis of variance (ANOVA), and means were separated using the New Duncan’s Multiple Range test performed with statistical package for Social Sciences (SPSS) 16.0 Software (SPSS, Inc. 2007).

Acknowledgment

The authors are grateful to Dr. O. A. Obembe of the Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria for his assistance in identification of plants used in this study.

Reference

Abbott W.S., 1925, A method of computing the effectiveness of an insecticide, Journal of Economic Entomology,18: 265-267

Akinkurolere R.O., Adedire C.O., and Odeyemi O.O., 2006, Laboratory evaluation of the toxic properties of forest Anchomanes, Anchomanes difformis against pulse beetles Callosobruchus maculatus (Coleoptera: Bruchidae), Insect Science,13: 25-29
http://dx.doi.org/10.1111/j.1744-7917.2006.00064.x

Akinkurolere R.O., Adedire C.O., Odeyemi O.O., Raji J., and Owoeye J.A., 2011, Bioefficacy of extracts of some indigenous Nigerian plants on the developmental stages of mosquito (Anopheles gambiae), Jordan Journal of Biological Science, 4(4): 237-242

Al-Dakhil M.A, and Morsy T.A., 1999, The larvicidal activities of the peel oils of three citrus fruits against C. pipiens, Journal of Egypt Social and Parasitology,29: 347-352

Amusan A.A.S., and Okorie T.G., 2002, The use of piper fruits oil as protectant of dried fish against Dermestes maculates, Global Journal of Pure and Applied Science, 8(2): 197-201

Ashamo M.O., Odeyemi O.O., and Ogungbite O.C., 2013, Protection of cowpea, Vigna unguiculata L. (Walp.) with Newbouldia laevis (Seem.) extracts against infestation by Callosobruchus maculatus (Fabricius), Archives of Phytopathology and Plant Protection,46(11): 1295-1306
http://dx.doi.org/10.1080/03235408.2013.765136

Begum N., Shaarma B., and Pandey R.S., 2003, Caloptropis procera and Annona squamosa: Potential alternatives to chemical pesticides, British Journal Applied Science and Technology, 3(2): 254-267
http://dx.doi.org/10.9734/BJAST/2014/2205

Forim M.R., Da-silva M.F.G.F., and Fernandes J.B., 2012, Secondary metabolism as a measurement of efficacy of botanical extracts: The use of Azadirachta indica (Neem) as a model, Insecticides-advances in integrated pest management, Dr. Farzana Perveen (Ed.), pp.367-390

Ileke K.D., and Oni M.O., 2011, Toxicity of some plant powders to maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae) on stored wheat grains, African Journal of Agricultural Research, 6(13): 3043-3048

Ileke K.D., and Olotuah O.F., 2012, Bioactivity Anacardium occidentale (L) and Allium sativum (L) powders and oil Extracts against cowpea Bruchid, Callosobruchus maculatus (Fab.) (Coleoptera: Chrysomelidae), International Journal of Biology,4(1): 8-13

Ileke K.D., Bulus D.S., and Aladegoroye A.Y., 2013, Effects of three medicinal plant products on survival, oviposition and progeny development of cowpea bruchid, Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae] infesting cowpea seeds in storage, Jordan Journal of Biological Science, 6(1): 61-66
http://dx.doi.org/10.12816/0000260

Isman, M.B., 2000, Plant essential oils for pest and disease management, Crop Protection, 19: 603-608
http://dx.doi.org/10.1016/S0261-2194(00)00079-X

Jeyabalan D., Arul N., and Thangamathi P., 2003, Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, Anopheles stephensi, Liston Bioresources and Technology, 89: 185-189
http://dx.doi.org/10.1016/S0960-8524(03)00036-1

Jenson T.G., Palsson K., and Borg-Karlson A.K., 2006, Evaluation of extracts and oils of mosquito (Diptera: Culicidae) repellent plants from Sweden and Guinea-Bissau, Journal of Medical Entomology, 43:113-119
http://dx.doi.org/10.1603/0022-2585(2006)043[0113:EOEAOO]2.0.CO;2

Kaufmann C., and Briegel H., 2004, Flight performance of the malaria vectors Anopheles gambiae and Anopheles atroparous (CPDF), Journal of Vector Ecology, 29(1):140-153

Nathan S.S., Kalaivani K., and Murugan K., 2005, Effects of neem limonoids on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae), Act Tropics, 96:47-55
http://dx.doi.org/10.1016/j.actatropica.2005.07.002

Ogungbamigbe T., Ogunro P., Elemile P., Egbewale B., OlowuO., and AbiodunO., 2005, Presciption patterns of antimalarial drugs among medical practitioners in Osogbo Metropolis, South-West Nigeria. Tropical Medical Health,33: 201-208
http://dx.doi.org/10.2149/tmh.33.201

Oliver-Bever B., 1986, Medicinal Plants in Tropical West Africa, Cambridge University Press, Cambridge, United Kingdom
http://dx.doi.org/10.1017/CBO9780511753114

Philipson J.D., and Wright C.W., 1991, Antiprotozoal compounds from plants sources, Planta Medical, 57: 553-559
http://dx.doi.org/10.1055/s-2006-960230

Rehm S., and Espig G., 1991, The cultivated plants of the tropics and subtropics, Verlag Josef Margraf, Weikersheim, Germany

Sivagnaname N., and Kalyanasundaram M., 2004, Laboratory evaluation of methanolic extract of Atlantia monophylla (family: Rutacea) against immature stages of mosquitoes and non-target organisms, Inst Oswaldo Cruz, Rio de Janeiro, 99(1):115-118

SPSS Inc., 2007, Statistical package for social sciences, SPSS, Inc., Chicago, IL

Udo I.O., 2011, Potentials of Zanthoxylum xanthoxyloides (LAM.) for the control of stored product insect pests, Journal Stored Product and Postharvest Research, 2(3): 40-44

WHO, 1996, World Health Organization. Instruction for Determining the Susceptibility and Resistance of Mosquito Larvae to Insecticides, WHO/VBC/75.583, mimeographed document

WHO, 2010, World Health Organization, Malaria: fact sheets No. 94, April 2010, (Accessed May 25th, 2011 at http://www.who.int/mediacentre/factsheets/fs094/en/)

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