Xiaoqing Su^{1, 2} , Xiao Yang¹

1 Yingjiahang S.T.Co.LTD, Zhuhai 519060, Guangdong, China
2 Department of Biology, Guizhou Medical University, Guiyang 550004, Guizhou, China
Author    Correspondence author
Journal of Mosquito Research, 2017, Vol. 7, No. 4   doi: 10.5376/jmr.2017.07.0004
Received: 20 Feb., 2017    Accepted: 13 Mar., 2017    Published: 17 Mar., 2017

This article was first published in Journal of Guiyang Medical College in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Su X.Q., and Yang X., 2017, An observation on virulence of mosquito-killing fungus, Pythium guiyangense after rejuvenated through its host, Culex quinquefaciatus larvae, Journal of Mosquito Research, 7(4): 27-31 (doi: 10.5376/jmr.2017.07.0004)

To observe the virulence of mosquito-killing fungus, Pythium guiyangense Su (Pg) after being rejuvenated through its host, Culex quinquefaciatus larvae. Bioassay was conducted by adding Pg mycelia with its host C. quinquefaciatus larvae into disinfected plastic containers, recording numbers of dead and infected larvae, and calculating mortality rates and infection rates. Pg was re-isolated from infected larvae using KPYG2, SFE and water agar plates, and used to infect C. quinquefaciatus larvae again. The mortality rates and infection rates, specially infection rates in groups and in individual containers were calculated and compared. After several cycles of continuous rejuvenation, C. quinquefaciatus larvae infection rates by Pg increased markedly, including the highest group infection rates and the highest and lowest infection rates in individual containers, which were generally up to 40% or above, specially, infection rates of 100% were achieved in individual container. Rejuvenation through host is an effective way to increase virulence of Pg to mosquitoes.

Culex quinquefaciatus; Larva; Rejuvenation; Mosquito biological control; Pythium guiyangense Su; Virulence