Construction of RNAi Interference Vector of Aedes Mosquito V-ATPA Gene and Its Lethal Effect on Aedes Mosquitoes by Transgenic Chlamydomonas
1 Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou 571199, China
2 Institute of Tropical Bioscience and Biotechnology, Hainan key laboratory of functional components and utilization of Marine biological resources, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China
Journal of Mosquito Research, 2020, Vol. 10, No. 2
Received: 12 May, 2020 Accepted: 12 May, 2020 Published: 12 May, 2020
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This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Mosquito-borne diseases seriously endanger human life and health all over the world. Among them, the Aedes mosquito plays a very important role in the transmission of diseases. It is a vector insect of dengue fever, Zika virus disease, yellow fever, and Chikungunya disease. Therefore, the control of Aedes mosquito plays an important role in preventing and controlling the above-mentioned infectious diseases. In this study, an RNAi expression vector pMaa7IR / V-ATPAIR of the Aedes mosquito V-ATPA gene was constructed, and the Chlamydomonas reinhardtii was transformed by the glass bead method. The obtained transgenic algae strain was fed to Aedes larvae. The survival rate of Aedes larvae, the time and number of pupations, and the time and number of adult emergences all had a great impact. The V-ATPA RNAi transgenic algae strain has a certain lethal effect on Aedes mosquito. Because microalgae are natural food for mosquito larvae, they are widespread in nature. At present, chlorella, spirulina, Chlamydomonas, and other green algae microalgae can be produced on a large scale in the factory. The technology is mature and the production cost is low. It can be put into closed areas on a large scale for a long time to form dominant algae species. It provides a new idea for biological mosquito killing to prevent the spread of dengue fever, zika virus disease and other malignant infectious diseases.
Aedes mosquito; Chlamydomonas reinharditii; V-ATPA gene; RNAi
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Journal of Mosquito Research
• Volume 10