Subrata Mallick ^{1, 2} , Devaleena Mukherjee¹ , Goutam Chandra¹

1. Department of Zoology, Mosquito, Microbiology and Nanotechnology Research Units, Parasitology Laboratory, The University of Burdwan, West Bengal, India
2. Department of Zoology, Maharajadhiraj Uday Chand Women College, Burdwan, West Bengal, India
Author    Correspondence author
Journal of Mosquito Research, 2015, Vol. 5, No. 9   doi: 10.5376/jmr.2015.05.0009
Received: 04 May, 2015    Accepted: 08 Apr., 2015    Published: 23 Jun., 2015

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Mallick et al., 2015, Evaluation of Larvicidal Efficacy of Acetone Leaf Extracts of Annona reticulata Linn. Against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus (Diptera: Culicidae), Journal of Mosquito Research, Vol.5, No.9 1-7 (doi: 10.5376/jmr.2015.05.0009)

The present study was designed to examine the larvicidal property of acetone leaf extracts of Annona reticulata L. (A. reticulata), (family: Annonaceae) against 1^st to 4^th instars larvae of three dreadful mosquitoes namely Aedes aegypti L., 1762 (Ae. aegypti), Anopheles stephensi Liston, 1901 (An. stephensi) and Culex quinquefasciatus Say, 1823 (Cx. quinquefasciatus). Cent percent mortality was observed at 12 ppm concentration in 1^st, 2^nd and 3^rd instars larvae of Ae. Aegypti, where as 9 ppm concentration showed 100% mortality in 1^st, 2^nd and 3^rd instars larvae of An. stephensi after 72 h of exposure. At 6 ppm concentration, 100% mortality was observed in 1^st instar larvae after 24 h of exposure and in 2^nd and 3^rd instars larvae of Cx. quinquefasciatus after 48 h of exposure. In the 72 h bioassay experiment, LC₅₀ and LC₉₀ values progressively decreased with the increase in exposure period, having the lowest values at 72 h of exposure. Among the three mosquitoes, Cx. quinquefasciatus proved to be most susceptible to acetone leaf extract of A. reticulata. Some non target organisms were found to be non-responsive to the bioactive extract when exposed. So, acetone leaf extract of A. reticulata was proved to be an efficient larvicidal agent against 3 important vectors like Ae. aegypti, An. stephensi and Cx. quinquefasciatus.

Annona reticulata; Acetone extract; Aedes aegypti; Anopheles stephensi; Culex quinquefasciatus; Larvicidal Activity