Methodological Paper
Artificial Rearing Methods and Observations of Spodoptera litura on Soybean Plants 


Molecular Entomology, 2025, Vol. 16, No. 1
Received: 12 Dec., 2024 Accepted: 15 Jan., 2025 Published: 26 Jan., 2025
Under controlled artificial conditions, the growth cycle and behavioral characteristics of Spodoptera litura (striped stem borer) were observed with soybean plants as the sole feed. Experimental conditions: temperature around 25°C, relative humidity 23%, with a 12-hour light and 12-hour dark alternating cycle. It was observed that the duration from the pupal stage to the next generation pupation was about 45 days, with adult emergence taking about 15 days, mating and oviposition lasting about 2 days, egg hatching taking about 4 days, and the larval stage lasting 22 days, with the prepupal period lasting about 2 days. During the larval rearing process, providing sufficient space and food can reduce cannibalistic behaviors. Observing the reproductive organs of the pupae accurately distinguishes their sex. In environments with lower humidity, reproductive efficiency decreases, with the number of new individuals being only ten times that of the previous generation. Observing Spodoptera litura in an artificial environment provides basic data for biological research, contributing to green pest control strategies and resource utilization.
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